SUMO Protease (ULP1) (Active)
- Highly active cysteine protease derived from Saccharomyces cerevisiae
- Efficient cleavage of affinity purification tags, such as UBL protein and SUMO, from fusion proteins
- Specific recognition of SUMO's tertiary structure rather than amino acid sequence
Source:
Escherichia coli
Concentration:
Endotoxin Test:
<1 EU per 1 μg of the protein by the LAL method.
Unit Definition:
Purity:
>90% as determined by SDS-PAGE analysis.
Form:
Liquid.
Storage Buffer:
Stability & Storage:
This product is stable after storage at:
• -20°C or -80°C for 12 months under sterile conditions from date of receipt.
Avoid repeated freeze/thaw cycles.
Shipping Conditions:
Dry ice
Escherichia coli
Concentration:
5 U/μL
Endotoxin Test:
<1 EU per 1 μg of the protein by the LAL method.
Unit Definition:
One unit of SUMO Protease (ULP1) cleaves > 85% of 2 μg control substrate at 30°C for 1 h.
Purity:
>90% as determined by SDS-PAGE analysis.
Form:
Liquid.
Storage Buffer:
SUMO Protease is supplied in 25 mM Tris-HCl, 250 mM NaCl, 0.05% NP-40, 0.25 mM DTT, 50% glycerol, pH 8.0.
Stability & Storage:
This product is stable after storage at:
• -20°C or -80°C for 12 months under sterile conditions from date of receipt.
Avoid repeated freeze/thaw cycles.
Shipping Conditions:
Dry ice
Background Information:
SUMO Protease (ULP1, Ubiquitin-like-specific protease 1) is a highly active cysteine protease derived from Saccharomyces cerevisiae. It has often been used as a biotechnological tool for cleavage affinity purification tags such as ubiquitin-like (UBL) protein, and SUMO from fusion proteins. ULP1 protease specifically recognizes the tertiary structure of SUMO rather than an amino acid sequence. ULP1 protease has a His-tag for easy removal from a cleavage reaction by using nickel affinity resins. Notably, the cleavage reactions are available in a buffer containing 2 M urea.
- To optimize cleavage conditions, it is recommended to run preliminary cleavage reactions at a small scale.
- Dilute the target protein sample to 1-2 mg/mL with PBS solution.
- An effective general of the SUMO Protease (ULP1) protease: target protein ratio is 1U:2 μg.
- Reaction can be performed at 4°C-30°C. 4°C is recommended as the starting standard. Incubate the reaction mixture at 4°C for 16 hours or overnight.
- Determine cleavage level of the samples by SDS-PAGE analysis.
◼ SUMO Protease (ULP1) cleavage of control proteinThe ratio of ULP1 to target protein in digesting assay was 1U to 2 μg at 4°C overnight. SDS-PAGE analysis of target protein digested with ULP1.
- Once optimize for the cleavage condition, the cleavage reactions can be scaled up to cleave a large amount of the target fusion protein